| 摘要 |
<p>In an assay of the free portion of an analyte, e.g. thyroxine, in a biological fluid in which the analyte is present partly bound to natural protein binders, in which the free analyte and an analyte derivative compete for reaction with a specific binder, a differential blocking agent is used to reduce binding of the analyte derivative, but without reducing binding of the analyte, to the natural protein binders. When the natural binder is albumin, the differential blocking agent may have an affinity constant for albumin of about 10<4> - 10<6>. When the analyte is thyroxine, the differential blocking agent may be 5-sulphosalicyclic acid or a derivative thereof at a concentration of 5 x 10<-><5>M to 5 x 10<-><2>M.</p> |
