| 摘要 |
PURPOSE:To obtain the titled substance, by extracting an animal cartilage with an aqueous solvent, precipitating it with a hydrophilic solvent, separating a specific fraction. CONSTITUTION:An animal cartilage such as cartilage of bovine fetus, etc. is sliced, homogenized with an aqueous solvent (e.g., aqueous solution of salt, etc.), and fractionated with acetone (45-65wt% precipitated). The prepared precipitate is redissolved in the aqueous solution of salt, and separated by membrane fractionation (100,000-300,000 molecular weight). A substance having inhibitory activity against multiplication of endothelial cell of blood vessel is separated from it. Or, it is further adsorbed on diethylaminoethyl agarose (7-9pH), and eluted with a saline solution (0.25-0.6M). The substance is optionally purified by a protein purifying means such as dialysis, etc., lyophilized and can be preserved. EFFECT:An animal cartilage having low side effect owing to derivation from organism, obtainable in a large amount, is used as a starting raw material.
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