| 摘要 |
PURPOSE:To obtain a biosensor which can measure easily and quickly the specific component in a blood with high accuracy by providing a measuring electrode and counter electrode on an insulating substrate, coating the substrate with a prescribed reactive layer and porous filter layer and depositing an anticoagulant on one of the coating layers. CONSTITUTION:Platinum is embedded to an insulating (PVC) substrate 10 to provide a measuring electrode 11 and a counter electrode 12. A nonwoven nylon fabric 13 is installed to cover the electrode system. The fabric 13 carries glucose oxidase 14 as oxidation reduction enzyme and potassium ferricyanide 15 as an oxidation type dye to be conjugated to the oxidation reduction enzyme in a dry state after said fabric is impregnated into the soln. thereof. A filter layer 16 consisting of porous polycarbonate is installed onto the fabric 13. An anticoagulant (NaCl) is deposited on the layer 16. A blood is dropped to such sensor and the potential of the measuring electrode 11 is swept (0-0.5V) on the basis of the electrode 12, then the oxidation current is measured, by which the concn. of the glucose in the blood is detected. The specific component in the blood is thus easily and quickly measured with good accuracy. |
