| 摘要 |
PURPOSE:To obtain alpha-amylase activity in a short time in high accuracy, by using a specific oligosaccharide as a substrate, adding a test specimen containing alpha-amylase to it, treating simultaneously the test specimen with alpha-glucosidase to give a reaction product, measuring enzymatically the reaction product. CONSTITUTION:An oligosaccharide shown by the formula I (n is 3-6) or formula IIis used as a substrate, and a test specimen containing alpha-amylase is added to it. Simultaneously or after the addition, the test specimen is treated with alpha- glucosidase and/or beta-glucosidase to give glucose-6-phosphate or 6-phosphogluconic acid as a reaction product, which is measured. EFFECT:Measurement is possible without causing rise in a blank value and without being influenced by endogenous glucose and maltose at all.
|
