摘要 |
<p>A recombinat DNA vector comprises (a) a replication origin and a selectable marker (b), a nucleotide sequence containing a promoter and a transcription initiation site, the transcription initiation mucleotide being the first transcribed nucleotide, (c) a restriction endonuclease recognition sequence whose cleavage site is on the 3 min - side of the transcription initiation nucleotide downstream from the promoter, and (d) a fragment of DNA inserted at the cleavage site. The inserted DNA fragment may code for rDNA, tDNA or snDNA.</p> |