PROCESS FOR PREPARING L-LYSINE UTILIZING MICROORGANISM

摘要

L-lysine was produced by fermentation using a microorganism having lysine-analogue resistance, threonine-resistance and homoserine, leucine and valine triple auxotrophic nutrients. Corynebacterium glutamicam ATCC 13286 was mutagenized with NTG and selected on a minimal agar medium containing AEC 0.3%. The selected analogue- resistant mutant was mutagenized again at 40≦̸C for 30 min. The mutagenized strain was cultured at 45≦̸C for 5 days to select a thermo-resistant mutant having high lysine-producing ability. The corynebacterium mutant having various properties was fermented to produce L-lysine of 50g/L at 40≦̸C.