| 摘要 |
A process for the preparation of an aqueous composition of antihemophilic factor, having a concentration of about 20 to 45 units of antihemophilic factor per ml, a specific antihemophilic factor activity of at least 1 unit per mg protein, and being obtained from an aqueous solution of antihemophilic factor derived from human blood plasma, said aqueous solution having a concentration of about 5 to 15 units of antihemophilic factor per ml, and a specific antihemophilic factor activity of about 0.3 to 1.0 unit per mg protein, which comprises: (a) effecting about 2- to 10-fold concentration of the low concentrate solution, (13) (b) adjusting the pH of said concentrated solution of about 5.0 to 6.0, (16), (c) cooling the resulting solution to about 0 to 14 DEG C, (17) (d) removing the precipitate which forms at said pH and temperature, (18) (e) further clarifying the solution from step (d), (21) and (f) adjusting the pH to about 7.0. (22) |
