| 摘要 |
PURPOSE:To obtain L-aspartic acid efficiently, by transferring a hybrid plasmid having DNA carrying a genetic information of aspartase to a bacterium having high aspartase activity, released from catabolite inhibition, using this novel bacterium. CONSTITUTION:A DNA carrying genetic information of aspartase obtained from a bacterium such as Serratia marcescens Sr41, Serratia marcescens OUT8529, etc. belonging to the genus Serratia is integrated into a vector plasmid such as pACYC177, 184, etc., and the prepared hybrid plasmid is transferred to a strain, a host bacterium such as Serratia marcescens APc-494 (FERM-P 392), etc. belonging to the genus Serratia, released from catabolite inhibition. A culture solution of the bacterium thus transformed, its mold, or a treated material of it is reacted with fumaric acid and ammonia to give L-aspartic acid. The bacterium released from catabolite inhibition is obtained by subjecting a strain belonging to the genus Serratia having aspartase activity to variation and induction treatment. |
