| 摘要 |
PCT No. PCT/SE78/00019 Sec. 371 Date: Mar. 14, 1979 Sec. 102e Date: Mar. 14, 1979 PCT Filed: Jul. 13, 1978 Pub. No. WO79/00044 Pub. Date: Feb. 8, 1979. A method for the determination of the quantity and type of a first biologically active component in a sample which is added to a well in a liquid-saturated, porous matrix covering a thin layer of a second biologically active component bound to the solid surface of a carrier. A third biologically active component included in the matrix is capable of reacting biospecifically with the first biologically active component when the sample is allowed to diffuse or migrate electrophoretically in the matrix forming a zone containing a precipitate of the first and third biologically active components. This precipitate will absorb with biospecificity to the second biologically active component, bound to the solid surface of the carrier and is visualized in a suitable manner on the surface after removal of the porous matrix, e.g., as a change in the surface tension angle observed with the aid of vapor condensation on the surface. The second and third biologically active components or the second and first biologically active components may be identical as long as the remaining component is capable of reacting with both of the others. All three biologically active components may be antigens and antibodies. The carrier is suitably made of plastic material. |
