VERFAHREN ZUR BIOTECHNOLOGISCHEN HERSTELLUNG VON POLY-D(-)-3-HYDROXYBUTTERSAEURE

摘要

1. Process for the biotechnological preparation of poly-D(-)-3-hydroxybutyric acid by aerobic cultivation of a microorganism of the genus Alcaligenes in an aqueous nutrient medium which contains sources of assimilable carbon, nitrogen and phosphorus, as well as the amount of organic and inorganic trace nutrients necessary for the growth of the microorganism, characterized in that a strain of Alcaligenes latus is cultured with a supply of nutrients which is complete and optimal for the growth of the microorganism, under unlimited growth conditions, in the temperature range 36 to 42 degrees C, with a dissolved oxygen content of 25 to 50% of the saturation value for air, and at a pH of 6.5 to 7.5 under sterile conditions, and the PHB is isolated in a customary manner by extraction from the biomass which is obtained thereby.