| 摘要 |
PURPOSE:To separate and to purify a polymorphic type component of reverse transcriptase derived from AMV in good reproducibility, by dialyzing a dissolution fraction of reverse transcriptase, passing it through a column having polyguanylic acid agarose as an adsorbent, changing the concentration of NaCl, eluting it. CONSTITUTION:A dissolution fraction of reverse transcriptase obtained and purified using avian myeloblastosis virus (AMV for short) as a raw material is dialyzed, and passed through a column having polyguanylic acid agarose as an adsorbent. NaCl concentration of eluate is changed from low concentration to high concentration, and reverse transcriptase of alpha type, alphabeta type, and betabeta type having given concentration not overlapping with each other are eluted. |
