| 摘要 |
PURPOSE:To measure the concn. of iron in serum simply with good accuracy, by measuring the difference of absorbancies at a specific measuring wavelength and a reference wavelength. CONSTITUTION:A nitrosoaminophenol is mixed with a serum specimen as a color forming specimen and the absorbancy of an iron complex generated by a complex forming reaction is measured. Herein, a measuring wavelength is matched with the absorption max wavelength of the iron complex formed by the color forming reagent and a reference wavelength is selected from a wavelength range longer than the measuring wavelength and stable in a spectrum. That is, the measuring wavelength is selected from a range of 650-850nm and the reference wavelength is selected from a range of 750-1,000nm and the difference of the wavelengths is set to 10nm or more, pref., 50nm or more. |
