| 摘要 |
1,182,280. Extracting hydrophilic substances. E.I. DU PONT DE NEMOURS & CO. 12 May, 1967 [16 May, 1966], No. 22087/67. Heading C2C. A process for a hydrophilic substance, particularly adenosine triphosphate, from a material containing the hydrophilic substance bound or sequestered to a water-insoluble substance comprises preparing a mixture of the material with water and an organic liquid extractant, the water being partially soluble in the extractant and the extractant being present in an amount sufficient to dissolve part but not all of the water, whereby there are formed as separate phases an aqueous phase containing the dissolved hydrophilic substance and an organic liquid extractant phase, separating the phases and recovering the aqueous phase. The process is particularly applicable to hydrophilic substances found in living organisms, e.g. nucleotides, nucleosides, flavins or watersoluble cofactors. Specified extractants are 1-butanol, 2-butanol, t-butyl alcohol, n-amyl alcohol, t-amyl alcohol and 3-pentanol, anhydrous 1-butanol being preferred. Example describe the extraction of adenosine triphosphate from a culture of Escheriahia coli. |
