Biological detection process using polymer-coated electrodes

摘要

A method is described for the amperometric determination of the concentration in a biological sample of a constituent which is a substrate for an oxidase enzyme by (1) catalyzing the substrate with the enzyme, thereby consuming O2 and producing H2O2 and (2) measuring the O2 consumption or H2O2 production by means of an inert metal indicator electrode intimately coated with a thin film of a perfluorosulfonic acid polymer. A reaction chamber is described which provides means for contacting the sample with the enzyme and means for making amperometric measurements utilizing inert metal indicator electrodes coated with a thin film of a perfluorosulfonic acid polymer.