发明名称 |
Isolation of cyclase, epimerase and a ring expansion enzyme for producing unnatural cephalosporins |
摘要 |
The enzymes, cyclase, epimerase and a ring expansion enzyme, are isolated separately from a cell-free extract of a prokaryotic beta-lactam producing organism. The isolated enzymes are used to produce unnatural cephalosporins from polypeptide precursors. Isolation is carried out by adding ammonium sulfate to 40% saturation to the cell-free extract to precipitate contaminating proteins, adding ammonium sulfate to 70% saturation to the resultant supernatant to precipitate the enzymes, suspending the precipitated enzymes in a buffer, separating epimerase from the suspension by gel filtration, and separating cyclase and the ring expansion enzyme from each other by ion exchange chromatography.
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申请公布号 |
US4510246(A) |
申请公布日期 |
1985.04.09 |
申请号 |
US19820410302 |
申请日期 |
1982.08.23 |
申请人 |
QUEEN'S UNIVERSITY AT KINGSTON |
发明人 |
WOLFE, SAUL;WESTLAKE, DONALD;JENSEN, SUSAN |
分类号 |
C12N11/08;C12N9/00;C12N9/90;C12N11/18;C12P35/00;C12P35/06;C12P37/00;(IPC1-7):C12N9/00;C12N9/06 |
主分类号 |
C12N11/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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