Device for detecting antigens and antibodies.

摘要

Appts. for the determn. of antibodies or an antigenic or haptenic substance in a sample is described using urease-linked immunosorbent assay technique in which the binding of an antibody urease or antigen-urease conjugate to a solid phase is used to indicate the presence or absence of antibodies or antigenic or haptenic substance in the sample. The solid phase is contacted with urea as enzyme substrate and the presence of NH3 is determined with dibromo-o-cresolsulphophthalein (I). The appts. for use in detection or determn. of presence of antigenic or haptenic substance or antibodies in a sample comprises a number of tubular or capillary elements, each having antibodies or antigenic or haptenic substances attached to an internal surface. The device permits fluids to pass simultaneously or sequentially through the capillary elements. With the device the need for a number of separate tests to be performed for the determn. of antibodies etc. is avoided.