Molecular cloning and expression in industrial microorganism species.

摘要

Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing one or more copies of the gene expressing the polypeptide of interest are introduced into a compatible host, the transformed hosts treated to form protoplasts and then fused with protoplast acceptors prepared from industrial strains. Efficient transfer of the vehicle containing the genes of interest is achieved, with the resulting modified industrial strains being effective producers of the desired polypeptide product.