SIMULATED ACTIVITY OF PROTEIN A DISPLAYED BY LIGANDS ATTACHED TO A CELLULOSE BEAD SURFACE

摘要

A method and compound for the purification of proteins including the attachment of a non-peptidic, small compound which simulates the affinity of Protein A for immunoglobulins to a support matrix. Once attached on the support matrix, the resulting monochloro-triazine derivative is reacted with an excess of an amino compound at a higher temperature to achieve high levels of substitution. The resulting support matrix with ligand is useful in the affinity separations of antibodies. Further, a mercapto heterocyclic system ligand may be attached to the super matrix and useful in affinity separations of antibodies.