发明名称 |
PROCESS FOR PURIFICATION OF LIPOPROTEIN CHOLESTEROL FOR USE AS A CHOLESTEROL REFERENCE MATERIAL |
摘要 |
A method for the purification of lipoprotein cholesterol. The method involves the steps of adsorbing lipoprotein material on a silica adsorbant, eluting the adsorbed lipoprotein with water at a pH of about 10 to 11.5, adjusting the salt concentration to about less than 0.05M, heating the lipoprotein to about 50 DEG to 100 DEG C. for a period of about 5 minutes to 24 hours, adding an alkaline carbonate and alkaline earth salt to form a precipitate and removing the precipitate, adjusting the pH from about 6.5 to 9.0 and recovering purified lipoprotein cholesterol. Also a method for using the cholesterol as a cholesterol reference material. |
申请公布号 |
DE3069532(D1) |
申请公布日期 |
1984.11.29 |
申请号 |
DE19803069532 |
申请日期 |
1980.12.24 |
申请人 |
MILES LABORATORIES, INC. |
发明人 |
GIRGIS, MAKRAM MELEK;JACKSON, DAVID ERIC;MANNUZZA, FRANK JOSEPH |
分类号 |
C07J41/00;C12Q1/60;G01N33/50;G01N33/92;G01N33/96;(IPC1-7):01N33/92;01N33/96 |
主分类号 |
C07J41/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|