GRANULAR SUBSTRATE, SURFACE THEREOF IS GRAFTED, AND MANUFACTURE THEREOF AND ADSORBENT FOR AFFINITY CHROMATOGRAPHY CONTAINING SAID SUBSTRATE AND BIOLOGICAL USE THEREOF

摘要

1. A particulate support for the manufacture, by covalently bonding of a ligand, of adsorbents for affinity chromatography on a column, said support being essentially constituted by particles of a base polymeric material adapted for use in column chromatography and on which are grafted molecules of at least one unsaturated monomer capable of forming a copolymer with the base material and bearing at least one chemical function capable of forming, subsequently, under non-denaturing conditions, a covalent bond with the ligand used in the envisaged adsorption, characterized in that the molecules of unsaturated monomer are grafted essentially at the surface of the particules of the base polymeric material, the amount of grafted monomer possessing an accessible chemical function per unit of surface area (estimable monomer per unit of surface area) being from 10 to 500 mu g per cm**2 of surface area of the support, and the ratio of the amount of grafted monomer possessing an accessible chemical function to the total amount of grafted monomer (estimable monomer/fixed monomer) being from 0.05 to 0.5.