VERFAHREN UND REAGENS ZUR BESTIMMUNG DES HAEMOGLOBIN-HAPTOGLOBIN KOMPLEXES IN GEGENWART VON FREIEM HAEMOGLOBIN

摘要

The present invention provides a process for the determination of the hemoglobin-haptoglobin complex in the presence of free hemoglobin by utilization of the different peroxidate properties of free and of bound hemoglobin, wherein, for the selective inhibition of the peroxidase activity of the free hemoglobin, a detergent is added and the residual peroxidate activity of the reaction mixture is measured. The present invention also provides a reagent for carrying out this process wherein, besides the substances required for the determination of the peroxidase activity, it contains a detergent for the inhibition of the peroxidase activity of free hemoglobin. Furthermore, the present invention provides a process for determining the haptoglobin content of a sample, as well as a process for determining glycosilated hemoglobin in a sample.