Process for stimulating asexual propagation of Simmondsia chinensis tissue

摘要

The present invention embodies a process for mass propagation of plant tissue preferably of a Simmondsia chinensis, commonly known as Jojoba, and plant tissue having similar characteristics thereto. The process is directed to asexual reproduction of plantlets from tissue of immature and mature plants from a single piece of donor tissue and involves performing the steps thereon of: sterilization; chemical pretreatment with a cytokinin to increase the likelihood of tissue survival; washing again in sterile water; placing and retaining for a specified period the prepared tissue on first and second culture media to support and stimulate tissue and shoot development; separating the shoots by surgical division and transferring to a shoot multiplication medium for further development for a desired period; then transferring to a rooting medium for development into plantlets which are then transferred to a soil mix. The process of the present invention including the steps of the chemical pretreatment soak in a cytokinin of preferred concentrations; the use of certain chemicals in the culture media are complementary to the pretreatment soak and provide for an increase in plantlet survival and multiplication over former processes.