摘要 |
<p>1 or more polypeptides (I) exhibit a hand at a pt. corresp. to Mr ca. 92,000 or bands at pts. corresp. to Mr values of ca. 92,000, ca. 80,000 and ca. 79,000; of ca. 92,000, ca. 72,000 and ca. 71,000; or of ca. 92,000, ca. 80,000, ca. 79,000, ca. 72,000 and ca.71,000; when subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Coagulant has a specific conjulant activity over 1800 U/mg, the activity being shown over a continuous period of at least ca. 10 min.; and the coagulant exhibits human factor VIII:C antibody binding capacity. IgG monoclonal antibody is produced by a hybridoma formed by fusion of cells from a mouse myeloma and spleen cells from a mouse previously immunised with human factor VIII:C. Antibody reacts with human factor VIII:C and exhibits one of the following reactions. (a) Reacts with polypeptides of Mr 92,000, Mr 180,000 or greater and Mr 54,000 and does not react with those of Mr 44,000, 71,000, 72,000, 79,000, 80,000; (b) reacts with polypeptides of Mr 92,000, 100,000 or greater and 44,000 and does not react with those of Mr 54,000, 71,000, 72,000, 79,000, 80,000; (c) reacts with polypeptides of Mr 79,000, 80,000 and 100,000 or greater and does not react with those of Mr 44,000, 54,000, 71,000, 72,000, 92,000; (d) reacts with polypeptides of Mr 108,000 or greater but not with those of Mr below 108,000.</p> |