| 摘要 |
PURPOSE:To determine the concentration of isocitric acid dehydrogenase in a specimen, in high accuracy, in a short time, by inserting carbon dioxide electrodes in the flow-cell type determination cells placed before and after the enzymatic reaction tube, and measuring the difference of the carbon dioxide concentration before and after the enzymatic reaction. CONSTITUTION:The carbon dioxide electrodes 15 and 18 are inserted in the flow- cell type determination cells 14 and 17, respectively. A manganese chloride solution 11 and an oxaloacetic acid solution 12 are introduced into the flow path by suction, and the specimen 13 is introduced into the path. The concentration of CO2 in the solution is measured immediately after the introduction by the electrode 15 (the peak A), the solution is subjected to the enzymatic reaction in the enzymatic reaction tube 16 heated at 37 deg.C to generate CO2, and the concentration of CO2 in the solution is measured by the electrode 18 (the peak B). The output signals from the electrodes 15 and 18 are converted to the activities by the amplifier 19 and the arithmetic operator 20, and displayed on the display 21. The activity of isocitric acid dehydrogenase can be determined from the output difference between the peak B and the peak A, and the progress of the enzymatic reaction can be detected. |
