| 摘要 |
Defined sequence single strand oligonucleotides which have a length of fewer than 200 units, incorporate modified nucleotide units which are pyrimidine or purine-based, and which may have readily detectable reporter groups attached to substituents on the modified nucleotide units thereof. Each reporter group is attached to the base of the respective nucleotide unit at a sterically tolerant site thereon, exemplified by the C-5 position of pyrimidine-based nucleotides and the C-8 position of purine-based nucleotides. Such oligonucleotides are useful in identification, localization, isolation and/or detection of complementary nucleic acid sequences of interest in cellular or cell-free systems. Also disclosed is a process for the chemical synthesis of single strand oligonucleotides having a predetermined number of modified pyrimidine and/or purine-based nucleotide units in predetermined sequence, to the bases of which readily detectable reporter groups are attached at sterically tolerant sites through substituents thereon, either prior to or after incorporation of such units into the oligonucleotide chain. Novel nucleosides useful in the chemical synthesis of labeled, defined sequence single strand oligonucleotides are also disclosed. |
