| 摘要 |
PURPOSE:To easily determine the base arrangement of a DNA specimen with high accuracy, by fabricating the autoradiography of the predetermined separated and developed lines of the DNA specimen by using an accumulative fluorescent sheet in place of a radiation film while applying predetermined processing to said autoradiography. CONSTITUTION:Positional informations due to radioactive labels of specific split, decomposed and migratel lines 1)G+A, 2)T+C, 3)G and 4)C of a DNA specimen are transferred and accumulated to a light accumulative fluorescent sheet 1. In a pre-reading part 2, the sheet 1 is subjected to linear scanning by laser beam 5 deflected by a light deflector 7 while moved to the direction shown by the arrow 9 and the emitted exhaustive fluorescence is detected by a light detector 11. The output of the detector 11 controls an amplifier 24 and an AD converter 25 through a control circuit 13. The sheet 1 receives similar operation in a main reading part 3 while the detection output is processed with a processing circuit 26 through the amplifier 24 and the AD converter 25 and, by performing interpolation identification of the separated and developed lines 1)-4) by a reference line 0) due to the bonding thereof, base arrangement is easily determined with high accuracy. |
