AN IMPROVED APPARATUS FOR SEQUENCING PEPTIDES AND PROTEINS

摘要

An improved apparatus for sequencing proteins and peptides in a reaction chamber using a spinning cup by successive coupling and cleavage reaction steps wherein the reaction chamber is always subjected to positive pressure. The improvement involves the elimination of the use of a vacuum in the chamber for drying or enhancing reagent or solvent transfer. Throughout the coupling and cleavage steps the reaction chamber is subjected to a positive pressure by the positive application of an inert gas through the reaction chamber for reagent and solvent transfer, drying and removal of waste.