| 摘要 |
PURPOSE:To prepare a large amount of L-tryptophan, by cultivating a variant belonging to the genus Bacillus, having tryptophan analog resistance and reduced chorismate mutase, capable of producing L-tryptophan. CONSTITUTION:A bacterium belonging to the genus Bacillus, having tryptophan analog resistance, capable of producing tryptophan, as a parent strain, is subjected to common operation for induction of variation, a strain having low activity of chorismate mutase is selected among a mold solution subjected to variation treatment, to give Bacillus subtilis (FERM-P 6845) or Bacillus subtilis (FERM-P 6846). The strain is cultivated in a common medium at 5-9pH at 20-40 deg.C for 1-4 days aerobically, and a large amount of L-tryptophan is formed and accumulated. |
