| 摘要 |
PURPOSE:To enable exact measurement in a measuring method of total bilirubin using a stabilized diazonium salt by using diphiline as a reaction accelerator and using an org. acid for a buffer system. CONSTITUTION:A stabilized diazomium salt which is heretofore used is usable and the amt. of the diphiline to be used as a reaction accelerator is preferably 2-10g/dl, more preferably 4g/dl. The larger amt. results in much blank reaction and the smaller amt. in retarded acceleration of reaction. A preferred org. acid has strong buffer power at around 3pH and the acid having about 2-5pKa is preferable. Citric acid, succinic acid, maleic acid, tartaric acid, acetic acid and their salt and other org. acid are used. The pH of the buffer system is preferably 2-4, and around 3pH is best preferred. Excessively high pH results in poor stability and excessively low pH results in poor reactivity. |
