PROCESS FOR PRODUCING POXVIRUS RECOMBINANTS FOR EXPRESSION OFFOREIGN GENES

摘要

Methods and compositions are provided for the use of vaccinia virus or other poxviruses as vectors for expression of foreign genes. Expression of foreign genes is obtained by combining poxvirus transcriptional regulatory sequences with uninterrupted foreign protein coding sequences in vitro to form a chimeric gene. The chimeric gene is flanked by DNA from a non-essential region of poxvirus genome to provide sites for in vitro homologous recombination in cells previously infected with a genus of poxvirus.