发明名称 |
FIXATION OF NUCLEOPHILIC GROUP-CONTAINING COMPOUND |
摘要 |
The invention relates to a process for the immobilization of compounds comprising nucleophilic groups. According to the process of the invention a polymer comprising an amido group is swollen in a buffer. For this purpose any buffer is suitable which contains no amino, sulfhydryl and/or hydroxy groups in the molecule. To the swollen polymer a solution of a quinone - preferably p-benzoquinone - in a water miscible organic solvent is added and the mixture is activated at 273 DEG -343 DEG K. for 0.5-48 hours - preferably for 24 hours. The unreacted quinone is washed out with an aqueous solution of a water miscible organic solvent and thereafter water and/or a buffer. To the purified gel at a pH value between 3 and 11 - preferably between 6 and 8 - a buffered solution of a compound comprising a nucleophilic group is added, the suspension is incubated at 260 DEG -313 DEG K. for 24 hours, the gel is separated and the non-bound compound comprising a nucleophilic group is removed by washing. |
申请公布号 |
JPS5998045(A) |
申请公布日期 |
1984.06.06 |
申请号 |
JP19830152572 |
申请日期 |
1983.08.23 |
申请人 |
REANARU FUINOMUBEJISERUJIYAARU |
发明人 |
ZUZANNA ANTARU;EBA BERERU;RASURO BOROSU;IBAN DAROTSUI;MIKUROSU KARUMAN;IMURE SUTO;BERA SAYANI |
分类号 |
C08F220/56;A61K38/16;C07C67/00;C07C227/00;C07H3/00;C07H3/02;C07K14/76;C07K17/06;C07K17/08;C08F8/00;C12N11/08 |
主分类号 |
C08F220/56 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|