摘要 |
<p>An adsorbent, characterized by a carrier with covalently bound ligands consisting of a solid phase which is completely or partially penetrated by or surface coated with a hydrophilic, molecular, polymeric netting. The bound ligands consist of atom or atomic group Y and a group X located terminally from Y in the ligand, wherein Y and X are separated from each other by two consecutively bonded carbon atoms and wherein X is SO2, SO, S or Se, and wherein Y is S, N, NR or NR2, where R is hydrogen or alkyl. The solid phase is formed of particles preferably of smaller diameter than 1 mm, and the polymeric lattice is formed of a polyhydroxy polymer such as a polygalactane, e.g. agar or agarose, or a polyamide such as a cross-linked polyacryl amide or a derivative thereof. The ligand has the structure R1-X-CH2-CH2-S- where R1 is an arbitrary aliphatic, aromatic or heterocyclic group having for example the structure -(CH2)m-(CHQ)n-H wherein Q is H, OH or SH and wherein n = 1, 2 or a higher number and m = 0, 1 or a higher number. The adsorbent is prepared by first introducing thiol groups into the polymeric netting for subsequent alkylization with vinyl- beta -haloethyl sulfone, -sulfoxide or sulfide. The adsorbent can be used, with an excellent result, for the fractionation of biopolymers such as proteins, for example.</p> |