| 摘要 |
PURPOSE:To obtain a method for analyzing a mixture of the enantiomer of a compd. having a specific group bound to asymmetric carbon atom by using a filler prepd. by grafting an N-carbamoyl amino acid deriv. or O-carbamoyl oxy acid deriv. to an inorg. carrier having a hydroxyl group on the surface in a liquid chromatography. CONSTITUTION:Various grafting methods are usable in preparing a chromatograph filler. For example, the filler is obtd. by grafting the organo silane, which is obtd. by bringing the amino alkyl silane expressed by the general formula (II) into reaction with the N-carbamoyl amino acid or O-carbamoyl oxy acid expressed by the general formula (III) and is expressed by the general formula ( I ) to an inorg. carrier of silica gel or the like. The filter having the residual group of an optically active N-carbamoyl alpha-amino acid or the residual group of an O- carbamoyl alpha-oxy acid is filled in a column and is used as a stationary phase of a liquid chromatography. The sepn. and analysis of a mixture of the enantiomer of the compd. having the N=-CO-group, -CONH-group, -OCO-group, -OCONH- group, -NHCONH-group or =N-CO-NH-group bound to asymmetric carbon atom are accomplished with good separability in a short time. |
