Recombinant DNA containing a hepatitis B virus gene, mammalian cells transformed with the cloned viral DNA, and production of hepatitis B virus proteins.

摘要

Described is recombinant DNA comprising a Hepatitis B virus gene recombined with a vector containing the origin of replication of SV40 DNA inserted into a Escherichia coli plasmid and being preferably deficient in the region of inhibiting replication in mammalian cells, mammalian cells transformed with the recombinant DNA, and a method of production of Hepatitis B virus proteins, i. . HBsAg and/or HBeAg. These HISV proteins have the same immunological properties as those of the natural HBV proteins originated from human blood plasma and can be used for the preparation of HBV vaccines and diagnostic reagents.