| 摘要 |
PURPOSE:To prepare a thermally denaturated deoxyribonucleic acid BD-01 having high host-mediated antitumor activity, extremely low toxicity, and soluble in aqueous solvents, by centrifuging disintegrated cells of bacteria belonging to Bacillus genus, and heating the obtained nucleic acid fraction. CONSTITUTION:Cells of a bacterial strain belonging to Bacillus genus, e.g. Bacillus subtilis ATCC-6633, Bacillus natto IAM-1071, etc. are disintegrated and centrifuged, and the obtained extract free from the bacterial cell is treated directly with an organic solvent to obtain a nucleic acid fraction, or said cell- free extract liquid is added with a nucleic acid coagulant to obtain a precipitate containing a nucleic acid fraction. The obtained nucleic acid fraction is heated at about 80-120 deg.C to obtain a thermally denaturated deoxyribonucleic acid BD-01 having antitumor activity. |
