| 摘要 |
PCT No. PCT/DE80/00119 Sec. 371 Date Apr. 23, 1981 Sec. 102(e) Date Apr. 17, 1981 PCT Filed Aug. 12, 1980 PCT Pub. No. WO81/00622 PCT Pub. Date Mar. 5, 1981.Method and apparatus for molecular spectroscopy, particularly for the determination of products of metabolism. The absorption of infrared radiation by a specimen (2) which contains the substance to be determined is measured. The absorption is measured simultaneously at two different wavelengths ( lambda 1, lambda 2), the first wavelength ( lambda 1) being so selected that upon changes in concentration of the substance to be determined in the specimen (2) only a negligibly small change, if any, of the radiation absorption takes place, while the second wavelength ( lambda 2) lies in the region of a substance-specific absorption band of the substance to be determined. After measurement of the radiation intensities (I1 and I2) at the two wavelengths ( lambda 1, lambda 2) the signal is standardized by formation of the quotient (I2/I1). The method makes it possible to effect a quantitative reproducible measurement in the natural biological medium or in dialysates without pretreatment of the specimen and with only small amounts of substance. By suitable selection of the second wavelength ( lambda 2) a plurality of substances can be determined.
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