| 摘要 |
<p>PURPOSE:To prepare L-threonine, in high efficiency, by culturing L-threonine- producing bacteria belonging to Brevibacterium genus and obtained by cell fusion. CONSTITUTION:An L-threonine-producing bacterial strain requiring L-isoleucine and L-lysine, resistant to alpha-amino-beta-hydroxyvaleric acid and S-(2-aminoethyl) cysteine, belonging to Brevibacterium genus, and obtained by the cell fusion of an L-lysine-requiring strain and S-(2-aminoethyl)cysteine-resistant strain both belonging to Brevibacterium genus, e.g. Brevibacterium lactofermentum AJ 11788 (FERM-P No.6411), is inoculated in a nutrient medium and cultured under aerobic condition at 5-8pH and 29-37 deg.C for 1-4 days. L-threonine is separated from the culture liquid medium.</p> |
