发明名称 |
Recombinant DNA process utilizing a papilloma virus DNA as a vector |
摘要 |
A novel method and composition are provided for the replication and expression of exogenous genes in eukaryotic cells. A segment of a papilloma virus genome capable of extrachromosomal replication is linked to the foreign gene(s) using recombinant DNA techniques to provide a biologically functional replicon with a desired phenotypical property. The replicon is inserted into a eukaryotic cell by transformation, and the isolation of transformant provides cells for replication and expression of the DNA molecules present in the modified plasmid. The transforming region of the bovine papilloma virus provides a unique vector in that it provides both the capability of autonomous extrachromosomal replication but also the malignant transformed phenotype. Thus, genes which of themselves provide no selectable phenotypical property can be conveniently and efficiently introduced into eukaryotic cells and the transformants selected. The method is useful in that the foreign DNA is faithfully expressed and the gene products (proteins), such as pro-insulin (an insulin precursor) is synthesized.
|
申请公布号 |
US4419446(A) |
申请公布日期 |
1983.12.06 |
申请号 |
US19800221565 |
申请日期 |
1980.12.31 |
申请人 |
THE UNITED STATES OF AMERICA AS REPRESENTED BY THE DEPARTMENT OF HEALTH AND HUMAN SERVICES |
发明人 |
HOWLEY, PETER M.;SARVER, NAVA;LAW, MING-FAN |
分类号 |
C12N15/86;(IPC1-7):C12P21/00;C12P19/34;C12N15/00;C12N1/00 |
主分类号 |
C12N15/86 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|