| 摘要 |
<p>NEW MATERIAL:A substance having the following physical and chemical properties. Elementary analysis value (%): H 6.75, C 45.28, N 13.63. Specific rotatory power: [alpha]D<30>=-76.6. Solubility: easily soluble in water, 0.01N HCl and 0.01N NaOH. Color reaction: positive in biuret reaction, copper-Folin's reaction. Appearance: white powder, and its aqueous solution is colorless. Molecular weight: about 60 kilodalton. USE:Having lymphoma growth effect, useful for preparing a physiologically active substance (e.g., interferon) by an animal cell (e.g., namarupa cell, or melanoma cell). PROCESS:Grown bovine serum is gelled with glacial acetic acid, the gel is homogenized and centrifuged to give a supernatant liquid. The supernatant liquid is dialyzed to remove acetic acid, ultrafiltered, lyophilized, the dried material is suspended in a base medium for cultivating an animal cell, an active ingredient is extracted a soluble part is subjected to gel filtratin, and the active ingredient is purified to give the desired substance.</p> |
