| 摘要 |
PURPOSE:To precisely determine a high specific gravity lipo protein cholesterol under a supernatant condition separately from other low-specific gravity lipo protein without being influenced by an alcolbin acid under a serum, by a method wherein a specified quantity of Cu<2+> and Fe<3+> or Cu<2+> and Fe<2+> are contained in a settling reagent of a serum sample. CONSTITUTION:CuSO4 and Fe2(SO4)3 or CuSO4 are contained in a settling reagent, i.e., an aqueous solution cntaining a phosphotungstic acid, MgCl2 and NaCl, and being set to pH 7.6 by NaOH. This causes selective precipitating of chylomicron, a very low specific gravity lipo protein, and a low specfic gravity lipo protein, and precisely determines a high specific gravity lipo protein cholesterol without the use of an ascrobic acid under a serum or an expensive and troublesome ascorbic acid oxidase, entirely without being influenced by an ascorbic acid, and perfectly separately from other low specific gravity lipo protein. |
