| 摘要 |
PURPOSE:To enable the accurate quantification of proteins of various densities in a proportional response area of a photodetector such as a TV camera, by quantifying a large quantity of protein after a color reaction in a short time, while quantifying a very small quantity of protein after a color reaction in a relatively long time. CONSTITUTION:Blood protein is separated into a flat-plate shaped polyacrylamide gel by a method of two-dimentional electrophoresis (electrophoretic separation device 1). This gel is processed in a pre-dyeing processing tank 2, and then is immersed in a buffer water solution containing 3,3'-diaminobenzidine. Subsequently, water peroxide is added thereto to develop the color of a separated protein spot (color-developing tank 3). A prescribed time t1 later, it is taken out in an image reader (TV camera) 4, an information on the optical concentration of an electrophoresis-separated image is analyzed by an image-analyzing device 6, and thereby a relatively large quantity of protein component is quantified. The gel from which the reading of an image is ended is returned to the color-developing tank 3, and a color reaction is continued. Next, at a prescribed time interval, i.e. after a time t2, the gel is taken out likewise in the image- reading device 4, and a relatively small quantity of component is quantified accurately without an error. |
