发明名称 |
Process for isolating Cu,Zn-superoxide dismutase from aqueous solutions containing said enzyme together with accompanying proteins |
摘要 |
Cu,Zn-superoxide dismutase (SOD) is isolated from aqueous solutions containing said enzyme together with accompanying proteins by chromatography of the solution at a pH of 4.7 to 5.5 on a cation exchange resin of the same polarity as SOD in the pH range used. As cation exchange resin may particularly be used carboxymethyl celluloses, cross-liked dextrans substituted with carboxymethyl groups or sulfopropyl groups or cross-linked agaroses substituted with carboxymethyl groups. The process lends itself to use on an industrial scale and provides a high yield of pure SOD.
|
申请公布号 |
US4388406(A) |
申请公布日期 |
1983.06.14 |
申请号 |
US19810315392 |
申请日期 |
1981.10.27 |
申请人 |
DE FORENEDE BRYGGERIER A/S |
发明人 |
JOHANSEN, JACK T. |
分类号 |
C12N9/02;C12N9/08;C12N9/88;(IPC1-7):C12N9/02 |
主分类号 |
C12N9/02 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|