Method for culturing non-adhering mammalian cells.

摘要

Non-adhering mammalian cells are cultured in an aqueous culture medium in suspension at a partial pressure of dissolved oxygen in the range from 0.01 bars (atm) to 0.05 bars (atm) until the middle stage of the exponential growth phase, and at a partial pressure of dissolved oxygen of more than 0.01 bars (atm) after the middle stage of exponential growth phase.