摘要 |
PURPOSE:To achieve the screening of an antitumor polysaccharide simply and quickly, by bringing a diluted polysaccharide solution not containing endotoxin into contact with a hemocyte extract of a horseshoe crab. CONSTITUTION:This screening of a polysaccharide having an antitumor effect is characterized by selection of a polysaccharide indicating positivity in a Limulus test by bringing a diluted polysaccharide solution not substantially containing endotoxin into contact with a hemocyte extract of a horseshoe crab. Applicable polysaccharide includes those obtained from animals, higher plants and lichen, those obtained from bodies and culture solutions of mildew, bacteria and yeast and additionally, those obtained by oxidation, reduction or hydrolysis of these polysaccharides and those obtained by the hydroxyethylation and carboxymethylation thereof. The measurement of the positivity in the Limulus test is done by determining the extent of gelation of the hemocyte extract of the horseshoe crab or the activity of a coagulated enzyme. |