A method for determining bioactive substances.

摘要

An active membrane containing a reactive biofunctional molecule is coated on a substrate and the latter is contacted with a solution to be analyzed containing a complex conjugate of said molecule. The rate of complexion of said conjugate moiety with the membrane (which is a function of its concentration in the analyte) is measured by optical means and correlated with corresponding rates obtained from standard reference measurements, thus providing data for determining the unknown concentration of said conjugate moiety in the analyte.