Multi-layered element for quantititative analysis of immuno reactant

摘要

A multi-layered element for the quantative analysis of an immuno-reactant having a water-impermeable and light-transmissible support layer; a coloring reagent layer carried by said support layer and containing a hydrophilic polymer as a binder; and a reaction layer covering said reagent layer and made of a porous matrix. When the analyte immunoreactant is an antigen, said reaction layer contains the specific antibody for the antigen, the antibody being immobilized by a first water-insoluble carrier. When the analyte immuno-reactant is an antibody, said reaction layer contains the specific antigen for the antibody. The reaction layer contains an enzyme substrate immobilized by a second water-insoluble carrier different from said first water-insoluble carrier. The coloring reagent layer contains a detection reagent composition for coupling with an enzymatic reaction product produced by the reaction beween the enzyme-labelled complex of the immuno-reactant and the enzyme substrate immobilized by the second water-insoluble carrier. Upon coupling of the detection reagent composition with the enzymatic reaction product, a color is developed, the optical density of which is measured to determine the quantity of the analyte immuno-reactant contained in a liquid sample.