| 摘要 |
Micro-optical tomography is a technique for more fully utilizing the information conveyed by light in microscopy. This is accomplished with an objective lens imaging on a two-dimensional sensor, by scanning a parameter affecting the phase and amplitude of the reconstruction of the wavefront by the lens, such as focus, to form, with the scanned parameter as an axis, a higher dimensional image matrix, and operating on that image matrix with computer-aided image processing to extract information not visible in the fixed optical reconstruction of conventional microscopy. The reconstruction can form an optical dissection of a specimen, or an image of a dimensional object with all planes in focus simultaneously.
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