Blood fractionation improvement.

摘要

The extraction of Factor VIII, the antihaemophilic factor in blood, is difficult due to its instability and the presence of impurities. An initial Factor VIII containing aqueous solution such as blood plasma is purified by subjecting a FactorVIII containing aqueous migrant solution to continuous flow electrophoresis wherein flow takes place in an annular separation chamber and is stabilised by means of an angular velocity gradient; and collecting a separated Factor VIII component.

In order to separate the Factor VIII from albumin and fibrinogen, whilst obtaining good recoveries of FactorVIII (e.g. -60%), the migrant solution is prepared by precipitating FactorVIII from the initial solution using an organic liquid such as ethanol, and removing and redissolving the precipitate in an aqueous medium and adjusting the pH to be within the range of 7.5 to 8.6, preferably 8.3 to 8.6.