| 摘要 |
PURPOSE:To prepare the titled compound which has no erythropoiesis-inhibiting action, by adsorbing crude erythropoietin derived from healthy human urine with a weakly basic anion exchange material in the presence of an inorganic neutral salt, and eluting with a specific water solution. CONSTITUTION:Crude erythropoietin obtained from healthy human urine (hormone which specifically stimulates erythropoiesis and is an essential factor for the differentiation of the blood stem cell to erythrocyte) is treated with a weakly basic anion exchange material (e.g. diethylaminoethyl cellulose) under neutral or acidic condition in the presence of 0.1-0.2mol concentration of an inorganic neutral salt (e.g. NaCl, KCl, etc.), and the adsorbed erythropoietin is eluted with an aqueous solution of 0.5-0.7mol concentration of an inorganic neutral salt to obtain purified erythropoietin free from erythropoiesis-inhibiting activity. EFFECT:A highly pure erythropoietin which can be used in the medical use, can be separated from the urine of healthy man in high yield.
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