| 摘要 |
Steroidal alcs. were prepd. by Mycobacterium species capable of producing I or II when provided with a steroidal substrate. Thus, a substrate culture was prepd. by inoculating a nutrient medium of glucose 10, meat ext. 1.0, and peptone 1.0% with M. parafortuitum and incubating at 30≦̸C, 72hr. The seed culture was then inoculated into a prodn. medium contg. soybean meal 4, K2HPO4 0.2, MgSO4.7H2O 0.1, fish oil residue 2, NaNO3 0.2, and cholesterol which was incubated at 30≦̸C, 160 hr. Extn. of the resulting fermn. broth with EtOAc followed by filteration of the ext. gave a filtrate having the steroid compn. cholesterol 0.05, I 2.65, and II 0.22g.
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